At 0:03, The Nutshack theme is remixed in with the game music. At 0:10, we hear an audio clip of a slapping noise, which originally comes from a gay porno, which involve 2 men in Spider-Man costumes. Later at 0:15, "Crank That" is remixed in with the game music. At 0:33, the game music's mashed up with the song "It's Everyday Bro".
At 0:48, it's a reprise of the music.